Trigonelline, Stachydrine and Betonine, Betonicine and Choline are all alkaloids found within Betony. They are all Betains, a type of Zwitterion where a nitrogen or phosphorus atom has a positive charge. The negative counterpart is seldom shown - it could be any negative ion, usually the chloride ion since common salt, NaCl, is a ubiquitous source.

Trigonelline (N-methylnicotinic Acid, or Nicotinic acid betaine), is found not only in Betony, but also in Fenugreek seeds, Garden Peas, Hemp seeds, Oats, Coffee and Potatos amongst a few other plants. Trigonelline in coffee is said to impart coffee with some anti-oxidative properties and contribute to its aroma and slightly bitter taste. Trigonelline is also produced within the human body as a result of the metabolism of niacin (Vitamin B³ or Nicotinic Acid), after which it is excreted in urine.

Stachydrine is also present also in Yarrow, Motherwort, Alfalfa (Lucerne), Chrysanthemum and Citrus plants. It is the betaine of L-Proline.

Structurally, Betonine is almost identical to Stachydrine, but with an additional hydroxyl group attached to the ring.

Choline is just the cation (the part of the ion with positive charge). It can be paired up with any negative ion, but usually this is the chloride ion, making Choline Chloride. Acetyl Choline is another important molecule with the Choline backbone, and is usually found as the chloride, Acetylcholine Chloride. Choline is an essential nutrient for mammals and is usually grouped as belonging to the B-complex vitamins.